Elisa HIV1+2 Test

Model Number: ELISA HIV1+2 Key Specifications/Special Features: Instructions for use anti-HIV 1+2 ElisaAssay procedure1. Prepare reagents: dilute 1 volume of concentrated washing buffer (20×) with 19 volumes of distilled water, mix well2. Add samples: open the foil pouch and remove the microplate, set up 1 well as blank, 2 wells as negative control, 2 wells as positive controlAfter dispensing 50L of Sample Diluent , dispense 50L of sample or negative control or positive control to the respective wellsGently vibrating the plate3. Incubate: cover the microplate with plate cover and incubate the coated microplate in a thermostat-controlled water-bath or microplate incubator at 37℃ for 60 minutes4. Wash the plate: remove the plate cover, aspirate the contents of all wellsFill the wells with the diluted washing buffer (10-20 seconds to soak) then aspirate againRepeat the procedure for 5 timesMake sure that the rest volume is minimal, by tapping plate onto absorbent paper5. Add conjugate: add 100L of conjugate to each well (except the blank well)6. Incubate: cover the microplate and incubate the plate at 37℃ for 30 minutes7. Wash the plate: repeat the wash procedure as in step 48. Add substrate: add 50L of substrate solution A and 50L of substrate solution B to each well, mix wellCover and incubate at 37℃ for 10 minutes
9. Stop reaction: add 50L stop solution to each well, mix well
10. Read the absorbance at 450nm, if a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm
Results
1. For the assay to be valid, the mean OD value of negative controls must be less than or equal to 0.1 and the mean OD value of positive controls must be greater than or equal to 0.8
2. Cut off value = 0.1+NC
NC= the mean absorbance value for two negative controls
Important: if the NC is lower than 0.05, take it as 0.05
3. OD value of the sample ≥ cut off value, it is positive for anti-TP
OD value of the sample < cut off value, it is negative for anti-TP
Precautions
1. Allow all kit components to reach room temperature before use
2. Follow the direction insert to control the reaction temperature and time strictly
3. Do not mix components of different lot numbers to use
4. The kit should be store at 2-8℃, do not use kit components beyond their expiration date